Abstract:

Aim: was to assess biophysical parameters of a renal parenchyma at experimental acute and chronic renal insufficiency at rats.

Material and methods. Experiment was carried out on 36 rats of both sexes in mass of 180-250 g. The bioimpedance analysis of a renal parenchyma was carried out in intraoperative way on the 7th day after modeling of acute renal insufficiency and 2 months later after modeling of acute renal insufficiency, using «G. Greven's method». Absolute and relative indicators of electric impedance were investigated.

Results. Chronic renal insufficiency in contrast to the acute renal insufficiency is characterized not only by reduction of absolute indicators of renal parenchyma electric impedance, but also by change of relative indicators. Apparently, relative indicators of the bioimpedance analysis reflect change of the renal parenchyma functional status and absolute peculiarities of its morphological structure.

Conclusion. The analysis of results of a bioimpedansometriya in separate zones of a kidney, showed that in a normal kidney the electric impedance in various parts of body is non-uniform, however at renal insufficiency indicators of an impedance are leveled. This phenomenon is necessary for checking in clinical researches and, in case of confirmation of experimental results, it can be used as diagnostic criterion at statement of the diagnosis of acute renal insufficiency.

References 

1.    Smirnov A.V., Dobronravov V.A., Kajukov I.G. i dr. Rekomendacii Nauchno-issledovatel'skogo instituta nefrologii Sankt-peterburgskogo gosudarstvennogo medicinskogo universiteta im. Akad. I.P. Pavlova: opredelenie, klassifikacija, diagnostika i osnovnye napravlenija profilaktiki hronicheskoj bolezni pochek u vzroslyh. Nefrologija [Recommendation of scientific-research institute of nephrology, of St.Petersburg medical univercity named after I.P.Pavlov: definition, classification, diagnostics and major ways of prophylaxis of chronic renal diseases in adults. Nephrology.]. 2008; 12(2): 75-93 [In Russ].

2.    Tomilina N.A., Podkorytova O.L. Ostraja pochechnaja nedostatochnost'. Nefrologija i dializ. [Acute renal insufficiency. Nephrology and dialysis.] 2009; 11(1): 4-20 [In Russ].

3.    Golidorsky M.S., Liberbal W. Pathophysiology-ischemic acute renal failure. In: Atlas of Diseases of the Kidney. Ed. By R.W. Schreier. 1999; 460.

4.    Pal'cev M.A., Anichkov N.M. Patologicheskaja anatomija: Uchebnik [Pathological anatomy.]. V 2-h t. T. 2. Ch. II. M.: Medicina. 2001; 680 [In Russ]..

5.    Stecjuk E.A. Sovremennyj gemodializ [Modern dialysis]. - M.: MID, 1998; 356 [In Russ].

6.    John T., Dandirdas., Kunig P. et al. Neurological disease of Renal Failure. Neurology and general medicine. - 3rd ed. 2002; 131-142.

7.    Ermolenko V.M., Nikolaev A.Ju. Ostraja pochechnaja nedostatochnost': rukovodstvo [Acute renal insufficiency. Guide-book.]. M.: GJeOTAR-Media, 2010; 240 [In Russ].

8.    Rjabov S.I., Natochin Ju.V. Funkcional'naja nefrologija [Functional nephrology...]. S-Pb.: Lan', 1997; 304 [In Russ].

9.    Shejman D.A. Patofiziologija pochki [Pathophysiology of kidney]. Per. s angl. 2-eizd., ispr.- M.- S-Pb.: «Izdatel'stvo BINOM» «Nevskij dialekt», 1999; 260 [In Russ].

10.  Spencer J.A., Giussani D.A., Moore P.J., Hanson M.A. In vitro validation of Doppler indices using blood and water. J. Ultr. Med. 1991; 10: 305-308.

11.  Nagao M., Murase K., Saeki H. Pulsating renal blood flow distribution measured using power Doppler ultrasound: correlation with hypertension. Hypertens. Res. 2002; 25: 697-702.

12.  Hascaev B.D. Impedansnyj metod v mediko-biologicheskih issledovanijah i ego pribornoe osnawenie. Medicinskaja tehnika [Impedance method of medical-biological investigation and its equipment.]. 1996; 3: 34-40 [In Russ].

13.  Livenec V.P. Impedansometrija nebnyh mindalin kak sposob ob#ektivizacii klinicheskih form hronicheskogo tonzillita. Dis. kand. med. Nauk [Impedancometry of glands as a method of objectivization of clinical forms of tonsillitis]. M., 2006; 159[In Russ].

14.  Noskova A.P. Vlijanie furosemida i mannitola na vyzhivaemost' krys pri ostroj pochechnoj nedostatochnosti. Farmakologicheskaja reguljacija funkcii pochek [The influence of furosemid on survival rate of rats with acute renal insufficiency. Pharmacological regulation of kidneys.]. Pod red. A.A. Lebedeva. - Kujbyshev, 1981; 40-44 [In Russ].