Abstract:

The preclinical evaluation of the 0,5 M solution of the manganese(II)-DTPA [Mn(II)-DTPA] complex (mangapentetate) has been carried out in order to test the ability of Mangenese to be employed as substiute of potentially toxic Gadolinium in paramagnetic contrast agents for the MRI clinical routines. The toxicologic tests of the Mn(II)-DTPAwere carried out in mice, rats and rabbits. Saline phantoms served for calculation of the R1 -relaxivity of the Mn(II)-DTPA, in comparison to the Gd(III)-DTPA (Magnevist). Normal healthy rabbits (n = 12), healthy dogs (n = 5) and dogs with tumors (n = 5) served for quantification of imaging abilities of the Mn(II)-DTPA in vivo in animals. The LD50 in rats was over 10 ml/kg, essentially close to that one of Gd(III)-DTPA. The increase in intensity oftheTI-weighted images induced by addition of the Mn(II)-DTPA in phantom tests did not differ significantly from the values obtained with gadopentetate. Mn(II)-DTPA delivered prominent enhancement of normal kidneys in healthy rabbits as well as chest tumors in dogs. We conclude the mangapentetate can be employed as paramagnetic contrast agent in routine MRI studies and is worth clinical testing. 

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