Website is intended for physicians
Search:

 

Abstract:

Diffuse liver lesion is one of the leading positions in the structure of hepatobiliary pathology Dispite the large number of available diagnostic methods biopsy followed by histological examination is a «gold standart» untill now. Bioimpedancemetry is a potential method of evaluation of morpho-functional analysys of the organ.

Aim: was to clarify dependence of indices of an electrical impedance of liver tissue on its morfo-functional status and to define general valuation principles of absolute and relative measures of an electrical impedance of an organ in case of liver diffusion pathology in an experiment.

Metarials and methods: the experimental study was carried out on 66 Wistar rats 180-230 g. The diffuse liver failure was modeled by using 0,02% solution of N-nitrozodiethylamin with water that animals were drinking during all the period of experiment (120 days). Bioimpedancemetry and morphological investigation as a next step were performed on 14, 30, 60, 90, 120 days in parallel with biochemical blood analysis, sonography of the liver and morphological analysis.

Results: biophysical parameters of liver's parenchyma in cases of toxic hepatitis were characterized by increasing of absolute bioimpedance indices more than 50% and increasing of impedance dispersion coefficient of 16%. But in cases of drrhosis of the liver biophysical paremeters were characterized by decreasing of absolute indices of electrical impedance and increasing of heterogeneity coefficient of the liver of 50 to 100% at different measurement frequencies.

Conclusion: obtained data confirm the diagnostic value of bioimpedancemetry and give prerequisites for further studying of parameters of an electric impedance of liver parenchyma in clinical practice.  

 

References

1.     Shirokikh I.N., Mavlitova L.A., Tuev A.V., Khlynova O.V Diagnostika fibroza pecheni: ideal'ny li metody? [Liver fibrosis diagnosis: are methods perfect?] Permskiy meditsinskiy zhurnal. 2013; 30(3): 93-102 [In Russ].

2.     Yuschuk N.D., Znoyko O.O., Dudina K.R., Belyy PA. Problema virusnogo gepatita C v Rossijskoj Federacii. [The problem of viral hepatitis C in the Russian Federation.] Terapevticheskiyarkhiv. 2014; 86 (10): 77-81 [In Russ].

3.     Fedeli U., Avossa F., Guzzinati S., Bovo E., Saugo M. Trends in mortality from chronic liver disease. Ann. Epidemiol. 2014; 24(7): 522-526.

4.     Morozov S.V., Isakov V.A., Kaganov B.S. Sovremennye metody neinvazivnoj ocenki vyrazhennosti fibroza pecheni. [Modern methods of non-invasive evaluation of the severity of hepatic fibrosis. ] Infektsionnye bolezni. 2009; 7(4): 44-49 [In Russ].

5.     Saba L., di Martino M., Bosco S., Del Monte M., de Cecco C.N., Lombardo V., Piga M., Catalano C. MDCT classification of steatotic liver: a multicentric analysis. Eur. J. Gastroenterol. Hepatol. 2015; 27 (3): 290-297.

6.     Toosi A.E. Liver Fibrosis: Causes and Methods of Assessment, A Review. Rom. J. Intern. Med. 2015; 53 (4): 304-314.

7.     Beloborodova E.V., Beloborodova E.I., Purlik I.L., Kalacheva T.P. State of the liver at chronic hepatites of various etiology (according to data of morphological study of liver biopsy specimens). Klinicheskie perspektivy gastroenterologii, gepatologii. 2014; 1: 31-36 [In Russ].

8.     Kobyliak N., Abenavoli L. The role of liver biopsy to assess non-alcoholic Fatty liver disease. Rev. Recent Clin. Trials. 2014; 9 (3): 159-169.

9.    Nikolaev D.V., Smirnov A.V., Bobrinskaya I.G., Rudnev S.G. Bioimpedansnyj analiz sostava tela cheloveka. [Bioelectric impedance analysis of human body composition.] Moscow: Nauka. 2009: 392 p. [In Russ].

10.  Rodin A.V., Pleshkov V.G., Leonov S.D. Opredelenie zhiznesposobnosti kishechnika pri ostroj kishechnoj neprohodimosti v ehksperimente. [Determination of the viability of the intestine during experimental acute intestinal obstruction.] Vestnik eksperimentalnoy i klinicheskoy khirurgii. 2011; 4(1): 145-147 [In Russ].

11.  Panchenkov D.N., Leonov S.D., Rodin A.V. Razrabotka algoritma bioimpedansnogo analiza novoobrazovanij na modeli perevivaemoj opuholi RS-1 v ehksperimente [Development of bioimpedance analysis algorithm of tumors on an experimental model with transplanted RS-1 tumor.] Vestnik eksperimentalnoy i klinichesk,oy khirurgii. 2013; 6 (3): 287-291 [In Russ].

12.  Panchenkov D.N., Leonov S.D., Rodin A.V. Bioimpedansnyj analiz v medicine. [Bioimpedance analysis in medicine.] Patologicheskaya fiziologiya i eksperimentalnaya terapiya. 2014; 2: 80-86 [In Russ].

13.   Tornuev Yu.V., Koldysheva E.V., Lapiy G.A., Molodykh O.P., Balakhnin S.M., Bushmanova G.M., Semenov D.E., Preobrazhenskaya V.K. Bioimpedancemetry in the diagnostics of inflammatory process in the mammary gland. Bulletin of Experimental Biology and Medicine. 2014; 156 (3): 381-383.

14.   Abdullaev N.A., Balahnin S.M., Bushmanova G.M., Karpova A. A., Koldysheva E.V., Krinicyna Ju.M., Lapij G.A., Mzhelskaja M.M., Majborodin I.I., Manvelidze R.A., Preobrazhenskaya V.K., Senchukova S.R., Tornuev Y.V., Shelkovnikova N.V., Sholenberg E.V., Yakovleva A.Y. Kriterii differencial'noj diagnostiki novoobrazovanij, gastritov i yazvennoj bolezni zheludka po pokazatelyam bioimpedansa. [Differential diagnostic criteria of tumors, gastritis and gastric ulcer by bioimpedance measuring]. Fundamentalnie issledovaniya. 2015; 1: 1775-1779 [In Russ].

15.   Antonienko S.G., Berdinskikh I.K., Mishnaevskaya E.G. Rol' nekotoryh komponentov sistemy ciklicheskih nukleotidov pri gepatokancerogeneze, inducirovannom N-nitrozdiehtilaminom u krys. [The role of some components of the cyclic nucleotide system in hepatocarcinogenesis induced by N- nitrozdietilaminom in rats.] Eksperimentalnaya onkologiya. 1990; 12 (5): 18-21 [In Russ].

16.   Portyanaya N.I., Osipenko B.G., Moskadynova G.A., Novokhatskiy N.K., Guschina A.A., Dobrolyubova B.A., Chernyak Yu.I., Sokolovskiy V.V. K biohimicheskomu mekhanizmu toksicheskogo dejstviya gidrazinov. [Biochemical mechanism of toxic action of hydrazines.] Materials of the 1 Congress of Toxicologists of Russia. М. 1998; 306 [In Russ].

17.   Bogdanov N.A. Patologiya, klinika i terapiya porazhenij zhidkimi raketnymi toplivami. [Pathology, clinic and therapy of damages with liquid rocket fuel. Leningrad:] VMA. 1970: 152 p [In Russ].

18.   Avakyan A.Kh. Novye molekulyarnye kriterii ocenki toksicheskogo dejstviya proizvodnyh gidrazina. Aktivnye formy kisloroda kak klyuchevye agenty v mekhanizme toksichnosti. [New molecular criteria for assessment the toxic effects of hydrazine derivatives. Reactive oxygen species as key agents in the mechanism of toxicity.] Farmakologiya i toksikologiya. 1990; 53 (1): 70-73 [In Russ].

19.   Obraztsov S.A., Leonov S.D., Troitskiy Yu.V., Fedorov G.N. Ustrojstvo dlya izmereniya impedansa biologicheskih tkanej. [Device to measure biological fabric impedance. ]Patent RF, no 2366360, 2009.

20.   Smorodinov A.V., Leonov S.D. Elektrod dlya provedeniya bioimpedansometrii. [Electrode for performing electric impedancemetry.] Patent RF, no 2318435, 2008.

 

 

ANGIOLOGIA.ru (АНГИОЛОГИЯ.ру) - портал о диагностике и лечении заболеваний сосудистой системы